Active antithrombin glycoforms are selectively physiosorbed on plasma extracellular vesicles

Antithrombin (AT) is a glycoprotein produced by the liver and principal antagonist of active clotting proteases. A deficit in AT function leads to qualitative deficiency, challenging diagnose. Here we report that may travel physiosorbed on surface plasma extracellular vesicles (EVs), contributing form “EV-protein corona.” The corona enriched specific glycoforms, thus suggesting glycosylation play key role partitioning between EVs plasma. Differences glycoform composition separated from healthy deficiency-affected subjects were also noticed. This suggests deconstructing into its nanostructured components, as EVs, could suggest novel directions unravel pathophysiological mechanisms.